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KMID : 0380020160310040193
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Korean Journal of Biotechnology and Bioengineering
2016 Volume.31 No. 4 p.193 ~ p.199
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Development of Genome Engineering Tools for Metabolic Engineering of Butanol-producing Clostridium Species
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Woo Ji-Eun
Kim Min-Ji
Lee Ji-Won
Seo Hyo-Joo
Lee Sang-Yup
Jang Yu-Sin
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Abstract
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Global warming caused from the heavy consumption of fossil fuel is one of the biggest problems to be solved. Biofuel has been gained more attention as an alternative to reduce the consumption of fossil fuel. Recently, butanol produced from the genus Clostridium has been considered as one of the promising alternatives for gasoline, fossil based fuel. Nevertheless, the lack of the genome-engineering tools for the genus Clostridium is the major hurdle for the economic production of butanol. More recently, genome engineering tools have been developed for metabolic engineering of butanolproducing Clostridium species, which includes genome scale network model and genome editing tools on the basis of mobile group II introns and CRISPR/Cas system. In this study, the genome engineering tools for butanol-producing Clostridium species have been reviewed with a brief future perspective.
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KEYWORD
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Clostridium, Genome engineering, Butanol, Mobile intron, CRISPR
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